ที่มา:วารสาร คณะสัตวแพทยศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Quality of Bull Deep Frozen Semen Extended with Different Glycerol Concentrations: Evaluated by Computer Assisted Semen Analysis)

ผู้เขียน:Rapiphan Uavechanichkul, Jatuporn Pongpeng, Sinchai Wirojwutthikul, Lertchai Jintapitaksakul, Kunnasant Saikhun, Mukda Rattanapaskorn

สื่อสิ่งพิมพ์:pdf

Abstract

Quality of bull deep frozen semen extended with 87% and 99% glycerol in egg yolk tris extender with one-step and two-step dilution methods assessed by CASA (Computer Assister Semen Analysis) were compared. Semen of 20 Holstein-Fresian bulls aged 3-5 years were collected and frozen once a week for 3 weeks. The result showed the post-thaw sperm motility and progressive motility at 0 h from the 87% and 99% glycerol were not significantly different (P>0.05). The ALH (amplitude of lateral head displacement) and BCF (beat cross frequency) of the sperm from the extender using the 99% glycerol was significantly higher (P<0.05). However, after 5 h of incubation at 37?C the 87% glycerol gave significantly lower motility and progressive motiloty with higher VAP (average path velocity), VCL (curvilinear velocity) and ALH (P<0.05). The results of dilution methods did not differ significantly at 0 h. except at 5 h. the one-step method revealed significant higher motility (P<0.05. There were no effects of glycerol cencentration and dilution methods on post-thaw sperm head elongation, area, plasma membrane and acrosomal integrity (P>0.05). In conclusion, using 99% glycerol and one-step dilution methods gave a better semen quality.

Article Info

วารสาร คณะสัตวแพทยศาสตร์(Journal of Kasetsart Veterinarians), Volume 020, Issue 2, May 10 - Aug 10, Page 53 - 62 |  PDF |  Page